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Working with Excel: Calculations 
61 
By typing the equation 
sign [Shift =] in a cell, 
you enter the 
equation mode 
you can perform all 
different kinds of 
calculations: 
simple ones: with 
the mathematical 
operands (by clicking 
into the respective 
fields) 
more complex ones 
by activating the fx 
button (or the field 
showing the functions 
used lately) 
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Tricks: If you want to keep a field constant in an equation: type a „$͞ in 
between column-letter and row-number
62 
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Tricks: If you want to calculate a long column double click onto the 
lower right corner of the cell with the equation
63 
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You can perform frequently used calculations by using 
predefined Excel sheets (as templates) 
64 
Excel-templates on my website (under „For Scientists“): 
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Working with Excel-Graphs 
• Highlight a certain cell area  
- press „Strg͞ or „Ctrl͞ if you want to 
activate separate areas 
- include the title row (if you want 
an appropriate legend) 
• click the Insert tab and the desired 
graph type  
- note: don͚t use the „Line͞ type if 
you want to generate a normal x/y-
graph, but the „scatter͞ type with 
connecting line („Line͞  type 
generates equal x-axis distances 
regardless of the data) 
• Changes can be made  
by right-clicking 
or double clicking 
graph elements  
66 
Working with Excel-Graphs 
• Clicking on the graphs 
activates additional tabs 
(Design, Layout, Format) 
• Clicking on the „Design͞ 
(Entwurf) button allows 
changing the graph 
appearance by adding 
legends, axis titles etc. 
• Right-clicking on an axis 
allows switching to a 
logarithmic scale, adding 
grid lines etc. 
67 
Working with Excel-Graphs 
• Right-clicking on data points  allows 
adding a trendline 
68 
y = -1,847ln(x) + 12,334 
R² = 0,9648 
0
5
10
15
20
25
0,01
0,1
1
Achsentitel
Achsentitel 
Ct (or CP) 
Ct (or CP)
Log. (Ct (or CP))
0
0,5
1
1,5
2
2,5
3
3,5
4
control treatment
1
treatment
2
treatment
3
treatment
4
fold of control
Adding error bars 
• Graph toolbar: Layout 
> „Fehlerindikatoren͞ 
(Error bars) 
• Choose the last option 
(further error bar options) 
• Choose 
„ enutzerdefiniert͞ 
(Custom) and click the 
button next to it 
• This opens a menu with 
the plus-error bar field 
activated 
• mark the area in the Excel 
sheet, which contains the 
error bar data 
• do the same for the 
minus –error bar field 
(or copy the entry from 
the upper field into the 
lower field) 
Generating graphs with two y-axes 
70 
0
50
100
150
200
0
5
10
15
20
25
30
35
0
5
10
reaction product
min 
Enzyme 1
Enzyme 2
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