362    PerkinElmer          
create
49
FRAP 166, 167, 168
acquisition protocol
168
background correction
328
measurement item
333
normalization
329
photo-bleaching correction
329
references
330
FRAP acquisition
overview
166
preparing experiment
167
preparing for
167
FRAP acquisition example 168
FRAP analysis 328, 329
FRAP Preview 166
free flight camera control 231
freezing video preview 141
FRET 337
data acquisition
337
references
337
fret analysis 337
applying calculation
338
bleed-through constants
337
fret dialog 338
G
gamepad 231
genate colocalization 323
grid AF 196
grid confocal images
capturing multi-channel
201
capturing single channel
199
grid controls 196
grid modes 197
grynkiewicz equation 336
H
hand tool 64
hints
enabling and disabling
27
histogram 262, 274
I
ICS 27
Illumination correction 349
IM image 340
image menu 67
image sequence 33
create new
42
measure multiple
247
image sequences
capturing
141
image view
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September 2011  363
measurements
261
import
preferences
37
import command 44
important concepts 33
install
MicroPoint
205
installation
dongle
25
server systems
25
single license
25
starting after
25
intensity modulated images 340
intensity profile See line profile
intersect 284
invert 284
Iterative Restoration 27
iterative restoration
applying
347
description
343
J
join objects 299
joypad 231
joypad preferences 231
K
keyframe
create
72, 236
edit
72, 237
kymograph 268
L
lambda stacks 185
laser lines 117
laser power 148
lasso tool 65, 265
Library
opening
36
library
compact
51
creating
34
definition of
33
format
47
menu
48
organizing data in
47
selecting items
55
library thumbnails 48
library view 33, 47, 54
license server
change password
26
installing Volocity with
25
switching to individual license
26
switching to server
26
VB.NET PDF Page Insert Library: insert pages into PDF file in vb.
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364    PerkinElmer          
light path manager 121
light path settings 123
line profile 69
logs 143
M
magic wand tool 65, 265
magnifying tool 64
make measurement item 273, 297
make movie 74, 238
make volumes command 43
make well overlay 155
mean intensity 305
mean intensity of objects measured 313
measure
selected timepoints
273
measure and track multiple timepoints 251
measure channels
ratio
336
measure device latencies command 121
measure multiple image sequences 247
measure multiple timepoints 243
measure psf 345
sampling
353
measure single timepoints 241
measurement columns 298
measurement item 243, 273, 297, 333
measurement protocol 241
batch processing
296
clear, save, restore,export
295
measurement protocol tasks 276
measurement table 297
measurements
count points
263
definitions
303
draw lines
263
draw regions
264
export
301
feedback
301
feedback options
272
filter data
298
histogram
262, 274
image view
261
join objects
299
object feedback
270
view options
272
measurements menu 271
measurements view 261
feedback options
301
merge channels command 78
merge planes command 100
MicroPoint 205
minimum system requirements 25
move
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September 2011  365
folders
50
items
50
movie
clear
73, 238
make
74, 238
resize
73, 238
save and restore
73, 239
movie interface 71
multiple channels over time acquisition 210
multiple channels, multiple z-planes acquisition 212
multiple channels, multiple z-planes over time acquisition 215
multi-point acquisition example 153
N
Noise 27
noise 355
normalization
FRAP
329
normalizing data 309
notes view 82
Nyquist theorem 354
O
objective lenses 120
offsets 94
open
library
36
open objects 281
optigrid 188
calibration
192
capturing multi-channel grid confocal images
201
capturing single-channel grid confocal images
199
connecting manual focus model
190
connecting motorized focus model
189
grid controls
196
how to use
196
manual focus model preferences
192
motorized focus model preferences
191
structured light algorithm
356
troubleshooting grid imaging
203
troubleshooting installation and calibration
194
overlays 155
save and restore
157
P
page setup command 105
pan tool 64
parent ID 304
pausing acquisition protocol 141
PDM 324
photo-bleaching correction
FRAP
329
PhotoKinesis accessory 150
pivotal point of rotation 231
C# HTML5 PDF Viewer SDK to view PDF document online in C#.NET
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366    PerkinElmer          
point tool 263
points 158
inspect and edit
159
random
159
save and restore
161
scanning
163
preferences
after installation
26
for acquisition
111
import
37
joypad
231
optigrid manual focus model
192
optigrid motorized focus model
191
photokinesis accessory
150
UltraVIEW
145
print command 105
print preview command 105
product of difference from mean See PDM
programming acquisition See acquisition setup dialog
properties 49
protocols
setup and run
163
PSF 343
psf
calculate
344
measure
345
Q
QTVR movie 230, 239
quantitation
measure and track multiple timepoints
251
measure multiple image sequences
247
measure multiple timepoint
243
measure single timepoint
241
quit Volocity 30
R
ratio
acquisition protocol
177
preparing experiment
176, 177
preview
173
ratio preview 173
ratioing 334
apply calculation
334
measure channels
336
raw data view 298
reference channel 56, 70, 222, 225, 229
reference image 58, 62, 137, 222
reference image See reference channel
reference images 93
regions of interest See ROI
registration corrections 95
automatically create
96
inspecting
97
manually create
96
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September 2011  367
remove items command 51
remove noise command 102
remove noise from objects 281
renderers
selecting
218
rendering view 217
resample timepoints command 79
resize movie 73, 238
Restoration 27
restoration
data quality
343
types of
343
reveal in finder/explorer command 51
ROI 267
roi options 66
roi tools 64, 264
rotate slice tool 227
rotate volume tool 228
rules 137
S
sampling
depth of scan
353
sampling for deconvolution 353
sampling frequency 354
save and restore movie 73, 239
Save settings See Bookmarks
saving acquisition protocol 140
scan points 163
scanning 161
scatter plot 319
selecting camera input 125
selection tools 64, 65
separate color images 183
separate monochrome images 185
separate touching objects 282
serial ports preferences 111
session log 29
shading defects 91
show color reference command 68
show data command 83
show in library command 51, 82
show scale command 69
show time command 67
shutter 119, 131
shutter controls 119
shutter management See device latencies
single channel over time acquisition 209
single channel, multiple z-planes acquisition 211
single channel, multiple z-planes over time acquisition 214
single frame acquisition 209
single point acquisition example 128
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368    PerkinElmer          
size
changing
87
skeletal length 304
slice view 226
create data
229
slicing orientation 226
sliders 119
smooth zoom command 67
snapshots 83
software updates 31
automatic check
27, 31
license server
31
sort by command 83
spectral signatures
create from color
180
create from monochrome
182
separating color
183
separating monochrome
185
spherical aberration 355
spinning disk 344
spinning disk illumination 145
split multi-colored items command 99, 102
split volumes command 100, 102
spot bleaching 126
stage setup 153
starting Volocity 25
statistics 321
colocalization
321
status bar (Windows) 55
stitch 134
storing measurements 297
subtract 285
supported file formats 45
T
target channel 266
technical support 23
thresholds 320
thumbnails
in library
48
thumbnails command 48
tile channels 126
timelapse 132
timepoint sequence
add
72, 236
edit
73, 237
timepoints
absolute or relative
77
measure and track
251
measure multiple
243
measure single
241
resample
79
total number of objects measured 311
total number of objects measured per timepoint 312
September 2011  369
track ID 304
track multiple timepoints 251
track objects 287, 288
automatically join broken tracks
288
trajectory variation
287
track objects task 253
triggering events See rules
troubleshooting
grid imaging
203
optigrid installation and calibration
194
session log
29
TTL controls 120
U
UltraVIEW 144, 145, 147, 148
undo command 85
unlock codes 30
using the optigrid 196
V
vibration 355
video feedback 128
video preview 115
CCD active area
127
centering XY stage
126
contrast enhancement
126
cropping
126
freeze
141
intensity
128
signal to noise
128
video preview window 125
view
3D
217
analysis
308
chart
316
colocalization
259, 318
export as AVI or QuickTime movie
108
library
47, 54
measurements
261
notes
82
raw data
298
rendering
217
slice
226
xy stage
154
view options 70
roi settings
66
view tracks 299
visualization 217
bookmarks
232
rendering and 3D view
217
set pivot for rotation
231
Volocity
important concepts
33
quitting
30
volumes
370    PerkinElmer          
align
240
create
43
split
102
voxel spy 126
W
well plate 155, 160
wells
overlays
155
scanning
162
X
XY focus maps 160
XY stage 153
centering on video preview
126
xy stage example 153
XY stage view 154
xy stage view
add overlays
155
XY tiling See stitch
Z
zoom tool 64
September 2011  371
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