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33 
FIGURE LEGENDS: 
Figure 1. Flow chart showing the experimental design of the animal studies. Rats in sham group 
were operated at the ankle to remove the tendon graft and at the knee with joint capsule opened 
and closed. 
Figure 2. Histological scoring system for examination of ACL reconstruction in rat knee joint 
samples.   
a
: „„No degenerative change‟‟ was scored to samples that did not reveal any traits of 
matrix degeneration. „„Minimal degeneration‟‟ refers to a lack of cell infiltration into graft with 
reduced  collagen birefringence. Cell infiltration into graft with  a significant loss of collagen 
birefringence  was  scored  as  „„significant  degeneration‟‟  (<50  %  of  region  of  interest).   
Appearance of bone or adipose tissue inside graft with extensive degeneration will be scored as 
„„extensive degeneration‟‟ (>50 % of region of interest).
Graft  incorporation  (including 
Sharpey‟s fibre and fibrocartilage zone) was defined as direct fibrous connection between bone 
and  tendon  as  revealed  under  polarization  microscopy.  “Extensive  graft  incorporation”  was 
scored  if  the  fibrous  connection  occurred  more  than  50  %  of  the  graft  tunnel  interface; 
“significant graft incorporation” was scored if the fibrous connection was less than 50 % of graft 
tunnel interface. “Minimal graft incorporation” was defined as non
-fibrous connection between 
bone and tendon gra
ft. “No graft incorporation” was characterized by either an empty space or 
interface structure that did not connect to both graft and bone. 
c
“Significant  peri
-graft  bone 
Appendix IVA
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XDoc.PDF for .NET, providing C# demo code for inserting text to PDF file
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34 
in-
growth” was scored when significant reduction in tunnel size was observed as comp
ared to 
time zero  (data not  shown). Minimal  peri-graft  bone in-growth  was scored when new bone 
formation was observed around the bone tunnel but the tunnel size did not significantly reduce. 
“No sig
n of bone in-
growth” was scored when no new bone formation
was observed around the 
tunnel. The presence of peri-graft bone erosion or the presence of cysts (tunnel widening) was 
scored as the worst situation with respect to bone tunnel healing
Figure 3. Serum level of C-reactive protein (CRP) in ACL reconstructed rats as measured by 
ELISA. Elevation of CRP was significantly reduced in all vitamin C groups (p=0.029) at day 1 
post operation. 
Figure 4. (A) A-P knee laxity was restored in 3mg/ml vitamin C group. There was no significant 
difference  between  3mg/ml  vitamin  C  and  Sham  group  (p=0.108),  whereas  the  A-P  knee 
displacements in saline (p<0.001), 10 mg/ml (p=0.006) and 30 mg/ml vit. C (p=0.023) groups 
were significantly higher than the Sham group. (C) Femur-graft-tibia pull-out force exhibited no 
significant difference between groups. (B) and (D) showed the set-up for the laxity test and 
pull-out test respectively. The arrow indicated the direction of force applied. 
Figure 5. Bright field and polarized images of representative H&E stained sections showing the 
tendon graft-femoral bone tunnel interface in ACL reconstructed rat knee at (A-B) day 1, (C-D) 
day 4, (E-F) day 7, and (G-H) day 42 with 3mg/ml vitamin C (right panel) or saline (left panel) 
Appendix IVA
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35 
irrigation. 
A‟
-
H‟ are the polarized view
s of the corresponding bright field images. At day 1, graft 
was intact in both group (A, B), but more hematoma between graft and bone tunnel was found in 
vitamin C group (B). At day 4, graft degeneration was not found in both groups (C, D). At day 7, 
loss of collagen birefringence was found in both groups (E, F), but cell alignment was better in 
vitamin C group (F). At day 42, graft integrity was better preserved in vitamin C group (H). 
Figure  6. Gait analysis of ACL reconstructed rats at pre-injury, 4 weeks, and 6 weeks 
post-operation.  (C)  Swing  duration  ratio,  and  (D)  Limb  Idleness  Index  were  significantly 
elevated  at  4  weeks  but  not  6  weeks  post-operation.  Increased  percentage  of  rats  with 
asymmetric walking gait (with LII>1.3) was found in the 10 or 30 mg/ml vitamin C groups.   
Appendix IVA
C# PDF Password Library: add, remove, edit PDF file password in C#
This example shows how to add PDF file password with access permission setting. passwordSetting.IsAssemble = true; // Add password to PDF file.
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Figure 1
Click here to download high resolution image
Appendix IVA
C# PDF File & Page Process Library SDK for C#.net, ASP.NET, MVC
Read: PDF Image Extract; VB.NET Write: Insert text into PDF; Add Image to PDF; VB.NET Protect: Add Password to VB.NET Annotate: PDF Markup & Drawing. XDoc.Word
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C# PDF Text Extract Library: extract text content from PDF file in
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Figure 2
Click here to download high resolution image
Appendix IVA
VB.NET PDF File Compress Library: Compress reduce PDF size in vb.
Also able to uncompress PDF file in VB.NET programs. Offer flexible and royalty-free developing library license for VB.NET programmers to compress PDF file.
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VB.NET PDF insert image library: insert images into PDF in vb.net
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Figure 3
Click here to download high resolution image
Appendix IVA
C# PDF File Split Library: Split, seperate PDF into multiple files
page of your defined page number which starts from 0. For example, your original PDF file contains 4 pages. C# DLLs: Split PDF Document. Add necessary references
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VB.NET PDF File Merge Library: Merge, append PDF files in vb.net
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Appendix IVA
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Appendix IVA
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