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ITSP 6.0
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Section F
–
Budget for the Project
(Please provide full justifications for each sub-
item under the budget items “Manpower”, “Equipment” and “Other Direct
Costs”. The rationale behind any projected income or expenditure has also to be given. In case certain goods or services
are intended to be procured from one company/organization/individual, please provide the details, relationship between the
applicant(s) and the company/organization/individual (if any) and justifications for not following the open procurement
procedures set out in the “Guide to the Innovation and Technology
Fund”.
In
addition, quotation/price information should
be provided in justifying expenditure items of $500,000 or more.)
1.
Expenditure
(Please ensure that all expenditure items must be incurred between the commencement and completion dates of the
project.)
1.1. Manpower
Key
member
Post/Rank
No. of
staff
Duration
Monthly rate
or equivalent
Total
Justification
(A)
(B)
(C)
(A)*(B)*(C)
(man-month)
($’000)
($’000)
*
Research
Assistant
1.0
12.0
18.002
216.024
1
Sub-total (I):
216.024
1.2. Equipment
(For each sub-
item under “Equipment”, apart from providing justifications for its procurement, please also
state in the explanatory notes whether similar equipment is available for sharing within the applicant
organization or with other ITF recipient organizations, and if so, the reason why the existing equipment cannot
be used for this project.)
Key
equipment
Item
Quantity
Unit cost
Total
Justification
(A)
(B)
(A)*(B)
($’000)
($’000)
Sub-total (II):
0.000
1.3. Other Direct Costs
(In case external consultants are required for the project, please set out clearly in the explanatory note the
justifications for engaging the consultants and the expected time commitment of the consultants under the
project.)
Item
Quantity
Unit cost
Total
Justification
(A)
(B)
(A)*(B)
($’000)
($’000)
Experiment animals (8 week-old male SD rats)
230.0
0.018
4.140
2
Animal holding charge
1,874.0
0.004
7.496
3
Reagents (Sodium Ascorbate)
1.0
4.000
4.000
4
1
5% MPF included; salary preliminary set according to the CU Pay Sacle B 12(a). The main tasks of the Research
Assistant include handling of the biochemical experiments such as ELISA and the histological studies. He is also
responsible for performing in vitro stability tests of Vitamin C solution and the gait analysis experiments and
provide surigcal assistance for the animal experiments (tendon repair surgeries and ligament repair surgeries).
2
Laboratory animals are provided by CUHK Laboratory animal service centre (webpage:
http://www.lasec.cuhk.edu.hk/). The unit cost of one SD rat (week 6-12) is HKD 17.9, as described on the CUHK
LASEC webpage (http://www.lasec.cuhk.edu.hk/Animal/price%202009.pdf ). As LASEC is a centre set up by
CUHK to facilitate ethical, qualified and safe animal research, it is desirable to utilize this centre to conduct the
animal experiments. Since CUHK LASEC reviews the price of animals only once per year and as the project will
end within a single year, inflation will not affect this cost.The animals involved in the experiments are charged at
cost only, with no cost being general or administrative support in nature.
3
Animal holding charge is the cost charged by CUHK LASEC for animal bedding and feeding. The cost for
keeping rats is HKD 3.27 per cage per day, as described in the CUHK LASEC webpage
(http://www.lasec.cuhk.edu.hk/Animal/price%202009.pdf ). All rats will be kept two per cage for the first
post-surgery week and four per cage thereafter. As LASEC is a centre set up by CUHK to facilitate ethical, qualified
and safe animal research, it is desirable to utilize this centre to conduct the animal experiments. Since CUHK
LASEC reviews the price of animals only once per year and as the project will end within a single year, inflation
will not affect this cost. The animals involved in the experiments are charged at cost only, with no cost being
general or administrative support in nature.
Appendix V ITF proposal on vitamin C irrigation solution
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ITSP 6.0
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Vitamin C Assay Kit
2.0
4.000
8.000
5
Animal surgical materials
1.0
10.000
10.000
6
Ultrasound Imaging
1.0
40.000
40.000
7
ELISA immunodetection kits
3.0
10.300
30.900
8
Histology & Immunohistochemistry
1.0
42.100
42.100
9
Seminars press conferences/ Medical exposition/
Workshop
1.0
20.000
20.000
10
Design and printing of pamphlet for dissemination
1,000.0
0.010
10.000
11
Patent costs
1.0
80.000
80.000
12
Audit fee
1.0
5.000
5.000
13
Sub-total (III):
261.636
Total((I)+(II)+(III)):
477.660
2. Amount of Sponsorship
(Please provide proof of the sponsorship in the form of annex to this application.)
Sponsoring Organisation
Cash
Equipment
Consumables
Total
(in cash-equivalent)
(A)
(B)
(C)
(A)+(B)+(C)
($’000)
($’000)
($’000)
($’000)
Total:
0.000
3. Income
(Please set out all income, except sponsorship, in this section.)
Item
Total
($’000)
Total:
0.000
4. Administrative Overheads
(Please budget not more than 15% of the net of total expenditure, sponsorship and income in this section if this is a
proposal submitted by a local university.)
Item
Total
($’000)
Administrative overhead
71.649
Total:
71.649
5. Net Amount Requested from the Innovation and Technology Fund
4
For formulation of vitamin C irrigation solution.
5
For measurement of ascorbate
6
The cost includes consumables such as surgical blades, needles, anaesthesia and sutures.
7
The operational cost include maintenance(1 year), and consumables such as acoustic windows of the scanhead
and the coupling gel
8
The ELISA kits will be used to measure serum level of CRP and IL-6.
9
The cost will include consumables for general histology such as sectioning blades, glass slides and staining
solution. Consumables for immunohistochemistry include primary and secondary antibodies, blocking serum and
colour development reagents.
10
After patenting, international conference is targeted to disclose some of the publishable information. One
relevant is the ISL&T, which traditionally lasts for 2 days in Feb or March in the US. Registration fee is around
USD 250 ($1950). Subsidizing 1 member of the team for economic class air travel (1 return ticket) and 2 nights of
stay requires approximately $12000. In addition, to disclose our findings to the local community regarding the effect
of vitamin C on ligament or tendon repair, 2 members of the team may be subsidized to join the Annual Congress of
HKOA. Registration fee will require $4000 ($2000 each). Furthermore, CUHK joins the HK Medical Exposition
every year, and the involved research assistant will introduce and demonstrate the sample product. This activity will
have minimal cost (product sample). Allowing for a 10% buffer in case of adjustments in the registration or other
fees, the total cost is estimated: (12000+1950+4000)*110% =$19745, or approximately $20000.
11
It includes the cost of printing and design of the pamphlet to promote the vit. C irrigation solution
12
The patent cost will cover the fee for filing patents in US and China patent office
13
Fee for auditting
Appendix V ITF proposal on vitamin C irrigation solution
33
ITSP 6.0
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Total
Expenditure
Total
Sponsorship
Total
Income
ITF Funding
(net of overhead)
ITF Funding
(overhead)
Net Requested
Amount from ITF
(A)
(B)
(C)
(D)=(A)-(B)-(C)
(E)
(D)+(E)
($’000)
($’000)
($’000)
($’000)
($’000)
($’000)
477.660
0.000
0.000
477.660
71.649
549.309
Appendix V ITF proposal on vitamin C irrigation solution
35
ITSP 6.0
Page 23 of 25
Section G
–
Classification of the Project
(When an application is approved, the information in this section will be published at the ITF website
.
)
1. Research Theme
Regular
2. Project Type
Product/Process/Technology Development/Improvement
3. Technology Area
3.1. Primary Area
Biotechnology
3.2. Other Areas (if any)
3.2.1.
3.2.2.
3.2.3.
4. Industrial Sector
4.1. Primary Sector
Biotechnology
4.2. Other Sectors (if any)
4.2.1.
4.2.2.
4.2.3.
5. Other Attributes (if any)
(Please feel free to choose more than one attribute.)
5.1.
Environment-related
5.2.
Quality-related
5.3.
Particularly SME-related
Appendix V ITF proposal on vitamin C irrigation solution
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Section H
–
Attachments for the Project
Annex No.
Section No.
Paragraph No.
File Name
1
C1.1
1
Annex 1. Pamphlet short version.pdf
2
C1.1
2
Annex 2. ISLTXAbstract ACL.doc
3
C1.3
2
Annex 3. ISLTIXAbstract VitC.doc
4
C1.3
2
Annex 4. US vascular image.tif
5
C1.3
2
Annex 5. US vascular change chart.tif
6
C1.4
2
Annex 6. ORS 2010 abstract US.doc
7
C1.4
3
Annex 7. HKOA 2009 abstract vascular.doc
8
C1.4
4
Annex 8. Fu 2009 JNM.pdf
9
C1.4
4
Annex 9. Catwalk.jpg
10
C1.4
5
Annex 10. HKOA 2008 abstract adhesion.doc
11
C2.2
7
Annex 11. Ultrasound imaging.jpg
12
C2.2
18
Annex 12. Patent template.doc
13
C3.4
1
Annex 13. CU-InnoExpo07.jpg
14
F1.2
2
Annex 14. Catwalk quotation.xls
15
E
CV - Kai Ming Chan.doc
16
E
CV - Leung-Kim Hung.doc
17
E
CV - Shu-Hang Yung.doc
18
E
CV - Sai-Chuen Fu.doc
19
E
CV - Po-Yee Lui.doc
20
E
CV - Gang Li.doc
21
E
CV - Wai-Hang Cheng.doc
Appendix V ITF proposal on vitamin C irrigation solution
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I hereby declare that:
(a) all factual information provided in this application as well as the
accompanying information reflects the status of affairs as at the date of
submission. I shall inform the Secretariat of the Innovation and
Technology Fund immediately if there are any subsequent changes to
the above information; and
(b) the ideas of the proposed project are original without any constituted
or potential act of infringement of the intellectual property rights of
other individuals and/or organizations.
Authorized Signature
with Organization Chop:
Name of Signatory:
Position:
Name of
Principal Applicant Organization:
The Chinese University of Hong
Kong
Date (dd/mm/yyyy):
Appendix V ITF proposal on vitamin C irrigation solution
48
Page 1
GRF1
RGC Ref No.d01825
RESEARCH GRANTS COUNCIL
Application for Allocation from
the General Research Fund for 2013 / 2014
Application Form (GRF1)
Please read the Explanatory Notes GRF(2) (Aug 11) carefully before completing this form.
To safeguard the interests of the researcher and the institution, awardee institution bears the
primary responsibility for prevention, detection and investigation of research misconduct,
including but not limiting to misusing of funds, data falsification, plagiarism and self-
plagiarism. Concerning research grant applications, the institution is strongly advised to use
anti-plagiarism software before submitting the application to the RGC.
PART I SUMMARY OF THE RESEARCH PROPOSAL
[To be completed by the applicant(s)]
1. Particulars of the Project
(a) (i) Name and Academic Affiliation of Principal Investigator:
Name
Post
Unit/ Department/ Institution
Prof Chan, Cavor Kai-ming Professor
(Clinical)
Dept of Orthopaedics &
Traumatology/The Chinese University
of Hong Kong
(ii) Is the PI a new appointee within 2 years of full-time paid appointment to his/her first
substantive position as an academic staff in a university at the time of submission of the
proposal?
Yes
No
(iii) Title of Project:
A novel approach to tissue engineering in ACL surgery and healing -
bioactive coating for controlled release of GHK tripeptide
(iv) Nature of Application
New
Re-submission
Continuation
(b) (i) Primary Field:
Orthopaedics/Traumatology
& Code
1224
Secondary Field:
Regenerative Medicine
& Code
1239
(ii) A maximum of five keywords to characterise the work of your proposal
(a maximum 30 characters for each keyword)
Appendix VI GRF proposal on GHK controlled release
45
Page 2
GRF1
RGC Ref No.d01825
1) Anterior cruciate ligament 2) sport injuries 3) tissue remodeling 4) controlled
drug delivery 5) GHK-Cu
(iii) Project Duration:
24
Months
(iv) Total cost of the project:
HK$
688,000
Less :
Other research funds secured from other sources
HK$
0
Amount requested : HK$
688,000
(c) Abstract of Research comprehensible to a non-specialist [a maximum of one A-4 page in
standard RGC Format for attaching PDF documents or a maximum 400 words for direct
input in the text box]:
Anterior cruciate ligament (ACL) rupture is a very common sport injury resulting in an
unstable knee. As a ruptured ACL does not heal, ACL reconstruction (ACLR) is the
standard treatment to restore knee function. The surgical procedure of ACLR involves
the use of a tendon graft to replace the ruptured ACL. Surgeons drill bone tunnels on
distal femur and proximal tibia, insert the tendon graft through the bone tunnels and
secure the graft in place. Poor graft healing is the major cause leading to ACLR failure
and excessive knee laxity, because the graft tissue is weakened with decreased
mechanical properties under hostile micro-environment at early healing phase, which
never return to the original level.
Glycyl-histidyl-lysine tripeptide copper complex (GHK-Cu) is a well-known activator of
tissue remodeling, which is used in many skin products. In the last GRF project, we
found that GHK-Cu can enhance graft incorporation and improve restoration of knee
laxity. However, repeated intra-articular injections of GHK-Cu at post inflammatory
phase may bring unnecessary tissue responses, and graft weakening at intra-articular
mid-substance was still evident. Therefore, we propose to develop a product to reduce
graft weakening in ACLR, by a bioactive coating on tendon graft with controlled delivery
of GHK-Cu. We have shown that a protective coating on tendon graft can reduce the
extent of graft deterioration at early stages after ACLR. In this research project, a
biodegradable thermo-sensitive hydrogel (which remains as liquid at room temperature
and forms gel at body temperature) will be developed to encapsulate GHK-Cu and apply
on tendon autograft or allograft to improve the healing outcomes of ACLR. As there are
over 1200 ACLR in Hong Kong per year, with over 200,000 ACLR in US per year and
probably millions in the world; our study will not just benefit the patients Hong Kong
but worldwide.
Appendix VI GRF proposal on GHK controlled release
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