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User 
Manual
FL
Fluorescence live cell movie analyzer
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Product components
Product overview
Installation
Description 
Operation 
Focusing 
Adjust the focus
Confluence
Bright image capture
Fluorescence image capture
Merged image capture
Monitoring 
Data 
Open data
Screenshot 
Save to USB
Rename
Delete
Counting
Sample-preparation
Semi AF
Bright counting 
Fluorescence counting
Save to USB
Settings
Set up parameter  
Control LED power
Setup date & time
Set up dilution factor
Set up image format
Update software & firmware
Check the disk space
PC software
Introduction 
Installation
Function guide
Home
Open data 
Dual project creation
Export data
Confluence
Edit movie file
Recount bright data
Recount fluorescence data 
Dual view
Cleaning & Maintenance
Troubleshooting 
Warranty 
Safety precautions
Product specifications
Ordering information 
Technical support
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Table of contents
JuLITM FL, Fluorescence live cell movie analyzer  ©2013 NanoEnTek Inc.
3
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Counting slide holder  
1 EA
Product components
JuLI™ FL is shipped with the following components. 
Please check all components listed below when an instrument was delivered.
If any item is missing or damaged, contact your local distributor or e-mail
sales@nanoentek.com.
· Model JULI-FGSC or JULI-FRSC <Scope unit> are only used in conjunction with the JULI-BFST <Station unit>.
Scope unit 
JULI-FGSC or JULI-FRSC
1 EA
USB drive 
4 GB
1 EA
Station unit
JULI-BFST
1 EA
User manual
1 EA
Power cord 
4 pcs/set, for U.S./Canada/
Taiwan/Japan, Europe or UK
1 SET
Quick  manual 
1 EA
Connection cable 
3 M
1 EA
Cell counting slide
(with 1.5 mL of trypan blue (0.4%))
1 Box
Cat No. JULI-BRS50
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Product overview
The JuLI™ FL uses states-of-the-art optics to get live-cell images from various cell 
culture dishes and can perform cell-based assays, including cell counting, cell viability, 
and quantitative analysis of GFP/RFP expression. It is able to detect the quantified 
cell confluence results with low variation and make a growth curve using image based 
analysis.
JuLI™ FL is able to capture sequential time-lapse images which can be converted to 
movie files (.avi) automatically. The compact design allows you to install the system 
in your cell-culture incubator easily. It can be used to compare the control and the 
experimental samples using the dual systems  (*optional) concurrently.
Features of JuLI™ FL, 
Fluorescence live cell movie analyzer:
Versatility
• Time-lapse image capturing & making a movie
• Cell counting and viability
• GFP or RFP expression level checking
• Dual system available for comparative cell analysis (*optional)
Accuracy
• Automated confluence detection & growth curve
• Semi auto-focusing with low variations
Ease of use
• Intuitive graphic user interface & LCD touch screen
• Compact size optimized for an cell culture incubator
• Simple steps for the system setups and the operations
• No calibration & maintenance required
JuLITM FL, Fluorescence live cell movie analyzer  ©2013 NanoEnTek Inc.
5
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Installation
Installing 
JuLI™ FL
Live-cell images from various cell culture dishes are directly 
captured in a cell culture incubator. 
This compact design allows an instrument to be installed in 
an incubator, and prevents the contamination by maintaining 
a sterile environment. Also, the intuitive graphic user interface 
provides quick and convenient use in a cell culture incubator 
while capturing time-lapse imaging.
IMPORTANT 
JuLI
FL is optimized into standard size of cell culture incubator.
It is recommend that the temperature should be stabilized in case of a small size incubator 
before using the instrument.
Avoid exposing JuLI™ FL to UV light. 
UV light may degrade components, including plastics. Damage from UV exposure is not 
covered under the manufacturer’s warranty.
Always wipe surfaces with ethanol-soaked paper towels. 
Do not directly spray ethanol anywhere on  JuLI™ FL. 
Dimensions of Scope unit
Width 
Depth
Height
Weight
Size
300 mm
190 mm
188 mm
4.5 kg
Station unit
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1.  Place the instrument on a flat, dry, and level surface after 
unpacking the instrument.  Allow at least 10 cm (4 inches) of free 
space at the back of JuLI™ FL for the proper ventilation and the 
prevention of overheating of electronic components.
2.  Plug the supplied power cord into the station unit of JuLI™ FL. 
3.  Connect a scope unit to a station unit using the supplied 
connection cable. 
(* For dual monitoring, the 2nd scope unit also should be connected to the station 
unit.)
4. Plug the power cord into the electrical outlet. Be sure to only 
use the power cord supplied with the instrument. Powering the 
instrument with an unapproved power cord may damage the 
instrument.
5.  When JuLI™ FL is ready to use, start JuLI™ FL by turning 
the ON/OFF switch on and pressing the Power button on the 
station unit. 
Installation
Installing 
JuLI™ FL
2.
3.
3.
IMPORTANT 
Connect to the 2nd
scope unit after connecting to the 1st scope unit completely. 
JuLITM FL, Fluorescence live cell movie analyzer  ©2013 NanoEnTek Inc.
7
Description
Front view
<Scope unit>
Focus knob 
: is used to adjust the image quality to obtain better cell images.  
Stage
: place sample here.
LED 
: JULI-FLG04 : Green, Bright
: JULI-FLR04 : Red, Bright
<Station unit>
LCD touch screen  
: Contains buttons for necessary functions and displays the images.
LED  
Stage
< Scope unit >
< Station unit >
Neck
Focus knob
LCD 
touch screen
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Description
Side view
<Scope unit>
Connection port   
: connects scope unit with station unit. 
<Station unit>   
Power button
: turn an instrument on and off by pressing the Power button.
USB Port 
: Connect a USB drive to save and transport images to PC.
Display port  (CRT port)
: connect to external monitor. (resolution: 1366 X 768 pixels)
: make sure to turn off an instrument before connecting.
< Scope unit >
< Station unit >
Connection port
USB port
Display port
Power button
JuLITM FL, Fluorescence live cell movie analyzer  ©2013 NanoEnTek Inc.
9
Description
Rear view
<Scope unit>
Fan
: is machinery cooling system.
<Station unit>
Power inlet
 connects JuLI
FL to an electrical outlet using the supplied 
power cord and the appropriate plug (based on the electrical outlet 
configuration in your country).
On/off switch
: is  main power switch. It is not necessary to use the on/off  switch for 
day-to-day operation of the instrument. 
Connection port 
: connects the scope unit with the station unit.
< Scope unit >
< Station unit >
Fan
Power inlet
On/off switch
Connection port
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