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3
Stage control
X-Y joystick - Top button toggles between very slow and
normal speed (beeps)
Focus
o
Manual coarse and fine knobs on each side
o
Away is up (see arrows above left knob)
o
You must manual focus the first time
Quick Focus
o
Auto up and down with buttons on right focus knob,
see arrows on scope
o
Always lower objective with down button before
placing or removing samples.
o
Up button returns to previous position
Do not use up button until you have found the focus manually.
Sample loading onto microscope
Put oil or water on objective (not 10x)
Put sample on stage, cover slip down, center with joy stick
Manually focus up (away) until immersion liquid contacts sample, lower slightly but don’t
lose contact with immersion liquid
Find focus through eyepieces.
To see your sample thru the microscope
Without using Zen
Brightfield
Touchscreen
Press ‘Set Work Position’ to get rid of this screen
Home-Make it Visible for viewing bright field
Look thru eyepieces and focus up
Wiggle the stage as you go, look for movement
Fluorescence
You must Start Zen software first, see next page
Once you set up for fluorescence, you can change the filters on the
microscope:
Fluorescence Filter Cubes:
Use 2 back buttons on left focus knob to change filters
Last button
o
Blue->green->red (towards red)
Second last button:
o
Red->green->blue (towards blue)
If spinning, hit same button twice
Note: Fluorescence at the microscope uses the Mercury-
Halide lamp, not the lasers. The Blue and Green filters
are Long Pass (LP) meaning you will see all colors through them.
These filters are not used for confocal imaging.
Make it Visible
for bright field at
the eyepieces
Right Focus Knob
Quick focus
s
Left Focus Knob
blue>green>red
red>green>blue
